Fig. 7: In situ hybridization of gene SOX2 in cross sections at 6 different time points post-amputation in the earthworm.

The slice size was 10μm. The 6 time points post-amputation included 0 (a), 6 (b), 12 (c), 24 (d), 48 (e) and 72 (f) hours. The red fluorescence represented positive signals and DAPI (blue fluorescence) was used to stain cell nucleus. Similar results in a–f could be ensured by three independently biological experiments.