Fig. 3: Heterogeneity of switched memory B cells is differentially represented in spleen and bone marrow.

Cells for single cell sequencing were FACSorted as IgG-expressing CD19⁺CD38⁺CD138^-GL7⁻ small lymphocytes. a Six transcriptionally defined clusters were identified by shared nearest neighbor (SNN) modularity optimization based clustering algorithm mapped to tSNE representation of spleen and bone marrow (BM) cells. tSNE coordinates and clustering was computed for 4754 from spleen and 2947 from BM cells, presentation is separated by organ. b Percentage of cells per cluster in each organ by mouse. c Distribution of IgG subclass mapped on tSNE. d Signature genes for each cluster, area under curve (AUC) of receiver-operator characteristics (ROC) of >0.7. e Distribution of transcription levels for representative genes mapped on tSNE. Cells for single cell sequencing were FACSorted as IgG-expressing CD19⁺CD38⁺CD138⁻GL7⁻ small lymphocytes from female C57BL/6 mice. Source data are provided as a Source Data file.