Fig. 5: Clonal trajectories connect predominantly clusters III and V of spleen and bone marrow.

a Percentage of shared clones between clusters of spleen and bone marrow. Clones were defined according to IgG heavy and light chain genes. Clones were randomly redistributed to clusters. Overlap significantly higher than random (P < 0.05, randomization test) indicated by red shading, white represents expected random overlap, and blue shading overlap lower than random, i.e., exclusive repertoires. b Mutation rates (percent of nucleotide sequence; upper panel) and CD80 (Cd80), CD73 (Nt5e), and PD-Ligand 2 (Pdcd1lg2) gene expression of individual cells. Cells for single cell sequencing were isolated by FACS as IgG⁺CD19⁺CD38⁺CD138⁻GL7⁻ small lymphocytes. c Frequencies of Bsm clones represented with higher mutation rates in clusters listed in rows, as compared to clusters listed in columns. Significant presence of clone members with additional mutations as compared to random distribution (P < 0.01, randomization test) is shaded in red. d Trajectories are based on accumulation of mutations within clones. Represented are significant trajectories (P < 0.01, randomization test). Arabic numerals indicate numbers of clones with additional mutations. Randomized redistribution of clones was performed 1000 times (a, c, d). Source data is provided as a Source Data file.