Fig. 4: Effect of substance P on the calcium signalling pathway in cultured adrenocortical cells.
From: The neuropeptide substance P regulates aldosterone secretion in human adrenals
a Effect of SP (from 10−12 to 10−6 M), angiotensin II (Ang II; 10−6 M) and ATP (10−5 M) on cytosolic calcium concentrations in cell pools (RFU relative fluorescence unit). Data are normalised to basal level (Bl) and expressed as mean of three culture dishes (triplicate). Recordings are representative of n = 3 independent cultures. b Maximum amplitude of Ang II- and ATP-triggered calcium responses (upper panel). Data are presented as mean ± SEM of eight cultures dished examined over three independent experiments. CI 95% were 154–197 and 129–160 for Ang II and ATP, respectively. Lower panel: dose-response curve of SP-induced maximum calcium responses normalised to those of Ang II illustrated in panel a.
