Fig. 7: Human oocytes do not exhibit detectable enrichment of Prc1 at kinetochores. | Nature Communications

Fig. 7: Human oocytes do not exhibit detectable enrichment of Prc1 at kinetochores.

From: Prc1-rich kinetochores are required for error-free acentrosomal spindle bipolarization during meiosis I in mouse oocytes

Fig. 7

a Prc1 localizes along spindle microtubules but not at kinetochores in human oocytes. MI oocytes of young women with no history of infertility were immunostained for Prc1 (green), kinetochores (ACA, red), microtubules (magenta) and DNA (DAPI, blue). Stages are categorized based on chromosome arrangement. Prc1 is detected at the spindle midzone after anaphase I. Oocytes from three women were examined. b Prc1 is not detectable at kinetochores. Human oocytes 5 h post NEBD were treated with the microtubule depolymerizer colchicine and immunostained for Prc1 (green), Ndc80 (red), and DNA (Hoechst33342, blue). Oocytes from more than three women were examined. Scale bars, 10 μm.

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