Fig. 1: Gene expression screen of PA-CF genomic scaffold KB871589.1. | Nature Communications

Fig. 1: Gene expression screen of PA-CF genomic scaffold KB871589.1.

From: A hypomorphic cystathionine ß-synthase gene contributes to cavefish eye loss by disrupting optic vasculature

Fig. 1

a Location of an eye QTL on A. mexicanus linkage group 21 (left), alignment to nearby scaffolds of the draft PA-CF (AstMex1.0.2) genome sequence based on the position of markers (center), and qualitative RT-PCR analysis of the expression of 21 predicted scaffold genes at 40 hpf (right). Expression of genes in SF and PA-CF are shown from top to bottom according to their 5’ to 3’ order on the scaffold. CM centimorgans, LG linkage group. See Supplementary Table 5 for PA-CF gene IDs. Genes downregulated in PA-CF compared to SF are shown in red. Information based on at least three biological replicates. b In situ hybridization showing hsf2bp expression in 40 hpf SF and PA-CF embryos at 25 °C (left) and after treatment at 37 °C for 1 h (right). Dashed lines encircle the eyes and lenses in the right frames. n = 20 for all frames. L lens, R retina. Scale bar: 200 μm; same magnification in all frames. Source data are provided in source data file.

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