Fig. 2: Expression of cbsa and cbsb genes during Astyanax development. | Nature Communications

Fig. 2: Expression of cbsa and cbsb genes during Astyanax development.

From: A hypomorphic cystathionine ß-synthase gene contributes to cavefish eye loss by disrupting optic vasculature

Fig. 2

a–p In situ hybridization showing cbsa (a–h) and cbsb (i–p) expression in SF and PA-CF embryos and larvae. b brain, e eyes, h heart p pectoral fin buds, l liver, m myotomes. n = 500 and at least 10 samples in each frame. q Sections through the heads of SF and PA-CF in situ hybridized larvae shown in g and h, respectively. ot optic tectum, cmz ciliary marginal zone of retina. Dotted lines: lenses. Top of frame is dorsal. r In situ hybridization showing cbsa expression in TI-CF, LS-CF, and MO-CF larvae at 40 hpf. Arrows: liver. Scale bar in a is 200 μm; magnification is the same in a–f and i–n. n = 140, 220, 240, 24, and 29 from top to bottom. Scale bar in g is 250 μm; magnification is the same in g, h, o, p, r. Scale bar in q is 300 µm; magnification is the same in both frames. s Qualitative (top) and quantitative RT-PCR comparing the levels of cbsa and cbsb expression in PA-CF heads and trunks. Graph: Bars are means and SEM of CF ΔΔCt values converted to percentage of SF ΔΔCt values (left y axis). Data points are ΔCt values (right y axis). Asterisk: significance calculated from ΔCt values (n = 3) by unpaired Student’s t test (p = 0.00). Source data are provided in source data file.

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