Fig. 8: Hypoxia in cavefish. | Nature Communications

Fig. 8: Hypoxia in cavefish.

From: A hypomorphic cystathionine ß-synthase gene contributes to cavefish eye loss by disrupting optic vasculature

Fig. 8

a Expression of hypoxia-sensitive genes by qPCR in isolated SF and PA-CF heads and trunks. Bars are means and SEM of CF 2−ΔΔCt values converted to percentage of SF values in three biological replicates. Data points are ΔΔCt values (n = 3). Asterisks: significance calculated from ΔΔCt values by unpaired Student’s t test (p = 0.000, 0.002, 0.002, and 0.002 from left to right). b, c Determination of hypoxia-inducing factor 1α (HIF1a) expression in SF (b) and PA-CF (c) eyes at 7 dpf by immunostaining. Dotted line surrounds SF eye. Arrow indicates the margin of CF eye. SF: n = 16. PA-CF: n = 16. Scale bar: 150 μm; same magnification in b and c. d HIF1α expression in CF eyes determined by immunostaining compared to optic growth during SF and PA-CF development. n = 16 larvae for each time point. Source data are provided in source data file.

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