Fig. 9: The expression of cbs genes and lens-associated phenotypes. | Nature Communications

Fig. 9: The expression of cbs genes and lens-associated phenotypes.

From: A hypomorphic cystathionine ß-synthase gene contributes to cavefish eye loss by disrupting optic vasculature

Fig. 9

a–f Apoptosis detected by Lysotracker staining in cbs morphant larvae and controls at 68 hpf. a SF control with no lens apoptosis (n = 14). b PA-CF control with lens apoptosis. (n = 26). cbsa (c) and cbsb (d) SF morphants with no lens apoptosis. (n = 22 and 14, respectively). e, f Apoptosis detected by Lysotracker staining in cbsa CRISPR-Cas9-edited SF larvae at 60 dpf: e SF control with no apoptosis (N = 24); f SF CRISPR-Cas9 with no lens apoptosis (n = 30). Dashed lines: outline of eyes. Complete lines: outline of lenses. g Lens removal from the optic cup on one side of SF larvae at 30 hpf (illustration of operation shown on top) did not induce optic hemorrhages at 4 dpf. (n = 23). Scale bar in a: 100 μm; Magnifications are the same in a–f. Source data are provided in source data file.

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