Fig. 1: Cryo-electron microscopy structure of the Csy–AcrIF9 complex.
From: AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex
a Model of the Csy–AcrIF9 complex, Csy subunits shown as pipes and planks, Cas6f shown in yellow, Cas7f in light blue and gray, Cas8f in purple, Cas5f in orange and crRNA in pink, AcrIF9 shown in red surface representation. b The binding site of AcrIF9.2, residues interacting with AcrIF9 highlighted in blue (Cas7.6f) and green (crRNA). Cas7f subunits shown in surface representation, crRNA and AcrIF9 in cartoon representation. c–e Detailed view of the AcrIF9 binding site, model shown in cartoon representation, interacting residues shown as sticks. Interactions with uridine 25 (U25) are expected to accommodate any base at the equivalent position. Hydrogen bonds are indicated by yellow dashes, hydrophobic interactions are indicated by blue dashes.
