Fig. 3: Molecular oscillation landscapes uncovered by iCMod.
From: Integrated omics in Drosophila uncover a circadian kinome
a Cycling mRNAs, proteins, and p-sites identified by iCMod vs. commonly used approach. b The enrichment analyses of 2768 transcriptionally rhythmic genes collected in CGDB23 and 1255 translationally oscillated mRNAs obtained from a previously reported ribosomal profiling against circadian mRNAs, proteins, and phosphoproteins detected by iCMod (two-sided hypergeometric test, from left to right, p value = 0.00001, 0.0614, 0.1299, 0.0013, 0.0305, and 0.1572; **p value < 0.01, *p value < 0.05). c Cycling mRNAs, proteins and NCPs identified in WT and per0 flies. d The enrichment ratio of NCPs that peak at a certain time point. e A hierarchical clustering of the NCPs ordered by the phase of the oscillation. Values of each NCP in all analyzed samples (column) are color coded based on the intensities. Colors indicate low (green) and high (red) Z scores. f The distribution of fold-changes for cycling proteins and NCPs. g The GO-based enrichment analysis of cycling mRNAs, proteins and NCPs (Two-sided hypergeometric test, p value of mRNA < 2.2 × 10−5, p value of Pro. < 1.4 × 10−4 and p value of Phos. < 3.1 × 10−7). h Western blots of proteins from whole-head extracts of WT and per0 collected at the indicated circadian time. ACTB was used as a loading control. SGG10 and SGG39 indicate different isoforms of SGG. Repeated five to seven times independently with similar results. i Quantification of SGG pY214 (n = 7), SGG protein (n = 5) and normalized SGG pY214 (n = 7) levels of blots in h. Error bars represent standard error of the mean (SEM). SGG pY214 of WT, one-way ANOVA, ***p value = 0.00033; SGG pY214, one-way ANOVA of WT, ***p value = 0.00002; ns, not significant. Source data are provided as a Source Data file.
