Fig. 2: Loss of MAFB limits β-cell derivation.
From: Loss of the transcription factor MAFB limits β-cell derivation from human PSCs
a Representative FC plots depicting the percentages of SOX17 + FOXA2+ cells and quantification at the DE stage (n = 6, biologically independent samples). b Representative FC plots depicting the percentages of PDX1 + NKX6.1+ cells and quantification at the PP stage (n = 6, biologically independent samples). c Representative FC plots depicting the percentages of GFP+ cells and quantification at the β-cell stage (n = 6, biologically independent samples). d Representative FC plots depicting the percentages of C-PEP + NKX6.1+ cells and quantification at the β-cell stage (n = 6, biologically independent samples). e Representative IF images from six independent experiments at the indicated stages of differentiation depicting SOX17 and FOXA2, PDX1 and NKX6.1, and C-PEP co-staining with PDX1 and NKX6.1. Scale bars, 50 μm. GFP and Brightfield images from live in vitro cell cultures. Scale bars, 100 μm. P values by one-way ANOVA followed by Dunnett’s multiple comparisons test were *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Data are presented as individual biological replicates and represent the mean ± SD.
