Fig. 5: MAFB is critical to β-cell identity.
From: Loss of the transcription factor MAFB limits β-cell derivation from human PSCs
a Representative Western blotting from three independent experiments for MAFB protein (37 kDa) expression in MAFB−/− and iHygroMAFB rescue cells with or without DOX as indicated. Vinculin (114 kDa) was used as a loading control. b Representative FC plots depicting the percentages of GFP+ cells and quantification at the β-cell stage (n = 3). P values by paired two-tailed t-test. c Representative GFP and Brightfield images from three independent experiments with or without DOX from live in vitro cell cultures at the β-cell stage. Scale bars, 100 μm. d The mRNA expression patterns of designated genes for islet hormones and selected transcription factors (INS, GCG, SST, PPY, PDX1, and MAFA) as measured by quantitative PCR analysis in the presence or absence of DOX (n = 3). P values by paired two-tailed t-test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. e Representative FC plots depicting the percentages of indicated markers at the indicated cell stages (n = 3 or 4, biologically independent samples for MAFB+/+, +/−, and −/− iPSC differentiation). f Quantification of the indicated cell populations from FC plots in (e), normalized to the unedited control, set to 1. g Representative IF images from three independent experiments at the DE, PP, and β-like cell stages of differentiation for the respective markers as indicated. Scale bars, 50 μm. P values by one-way ANOVA followed by Dunnett’s multiple comparisons test were *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
