Fig. 3: Thermogenesis is impaired in NCLX-null mice.
From: NCLX prevents cell death during adrenergic activation of the brown adipose tissue
a Survival curves of 8–10-week-old NCLX KO and WT male mice cold stressed at 4 °C. Animals reaching temperatures 28 °C and below were returned to room temperature for recovery and counted as drop-outs. Survival rates depict the rate at which animals had to be removed from 4 °C to room temperature to avoid mortality (n = 4 mice for WT and n = 6 mice for NCLX KO). CBT, core body temperature. b Core body temperature traces of the animals from experiment (a) during cold exposure (4 °C). The dashed red line indicates animal removal. c VO2 of NCLX KO and WT mice subjected to cold (4 °C) at t = 0 and t = 7 h (n = 5 mice per group). d–f Non‐shivering thermogenesis of NCLX KO and WT mice assessed by measuring O2 consumption at baseline and followed by β-3 adrenergic stimulation (CL-316,243 injection (1 mg/kg)) in anesthetized mice at 30 °C. d Traces of VO2 of NCLX KO and WT mice. e VO2 at baseline and under CL stimulation. f Fold increase of O2 consumption after the CL stimulation (n = 5 mice for WT and n = 6 mice for NCLX KO). g Survival curves of WT and NCLX heterozygous (+/−) littermates cold stressed at 4 °C; mice reaching 28 °C or lower were returned to room temperature for recovery and counted as drop-outs (n = 4 mice for WT and n = 9 mice for NCLX+/−). h Core body temperature of the animals shown in g. i VO2 of WT and NCLX+/− mice at basal and after CL-316,243 injection (1 mg/kg), under anesthesia at 30 °C. Traces of VO2 of NCLX+/− and WT mice. j VO2 at baseline and under CL stimulation (n = 4 mice per group). Student’s t-test (c, e, f, j); two-way ANOVA (d, i). Data are expressed as means ± SEM. nsp > 0.05, *p < 0.05, **p < 0.001, ***p < 0.0001. Replicates are indicated by individual dots shown for each group in all graphs. Source data are available as a Source Data file.
