Fig. 4: The japonica allele of OsSGR increases grain filling and yield when introgressed into elite indica cultivars.
From: Natural variations at the Stay-Green gene promoter control lifespan and yield in rice cultivars
a Whole-plant senescence phenotypes of an indica-type cultivar (IR72) and its near isogenic line (IR72-NIL) harboring the japonica OsSGR allele grown in paddy field. Scale bar, 20 cm. Color changes of the flag leaves (b) and panicles (c) from plants in (a) during seasonal senescence (1, 4, and 7 weeks after heading). Scale bar, 5 cm. In colourimetric assay, the same scale is used as in Fig. 1. d Senescence phenotype of a japonica cultivar (JN) and its near isogenic line (JN-NIL) harboring the indica OsSGR allele. Scale bar, 20 cm. Color changes of the flag leaves (e) and panicles (f) from plants in (d). Quantification of leaf color change in the flag leaves (g) and panicles (h). Data are means ± SE (n ≥ 4). i Temporal changes of chlorophyll levels of flag leaves from 1 to 7 weeks after heading. Data are means ± SE (n = 8). j Analysis of OsSGR expression in the flag leaves from plants in (a, d) from 2 to 7 weeks after heading. Data are means ± SE (n = 3). k The analysis of the net CO2 assimilation rate in flag leaves of IR72 and IR72-NIL during the grain-filling stage (4, 5, 6, and 7 weeks after heading). Data are means ± SE (n = 5). Total grain yields per plant (l) and grain-filling rates (m) of the near isogenic lines (NIL). In the box plots, the center value is the median, and the bottom and top edges of the boxes display 1.5 times the interquartile range. Values are the mean ± SE (n ≥ 16). *P < 0.05, **P < 0.01 by Student’s t test. Source data of Fig. 4g–m are provided as a Source Data file.
