Fig. 1: Human OMC generation and characterization.
From: The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culture
a Overview of the OMC generation (prior to immune cell addition). b Histological comparison of tissue sections obtained from primary tumour lesion (left) and OMC (right), showing epidermal and dermal compartments with interspersed tumour cells. Haematoxylin-eosin staining, representative pictures (n = 3). Representative areas (n = 3) of primary tumour lesion (left) and OMC (right), showing triplex fluorescent staining of Ki67 (magenta), tumour marker (yellow) and DAPI (blue) fluorescence IHC staining. Composite and single colour images of higher magnification are shown. Arrows indicate proliferating tumour cells. Representative flow cytometry histogram of Ki67+ cells measured within the OMC-digested live CD45-negative fraction. c Multiplex fluorescence IHC shows representative area (n = 3) of the tumour lesion (left) and the OMC (right) containing fibroblasts (fibroblast-specific protein 1, FSP1+ cells, magenta) and melanoma cells (tumour marker (tyrosinase and SOX10)+ cells, yellow). DAPI (blue) indicates nuclei. Inserts show higher magnification. Pseudo-DAB of isolated fluorescent channels of the same areas are shown and fibroblasts are indicated with arrows. Scale bars, 100 μm. Source data are provided as a Source data file.
