Fig. 2: The OMC microenvironment sustains immune cell survival and distribution.
From: The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culture
a Overview of the experimental approach used to obtain an immunocompetent OMC. b Tissue classification and DC localization within the selected ROI. Coloured dots indicate distribution of immune (red) and other cell types (pink), based on tissue segmentation and positivity score (upper panel, 1); cytometric image analysis and gating strategy to identify viable DCs (DAPI+ Cleaved-Caspase-3− CD45+ events) (lower panel, 2). c Viable cDC2s as a result of the gating strategy visualized in the original image, showing a representative selection of gated events. DAPI (blue), CD45 (green). d Quantification of viable, immune cells/mm2 in at least 5 sections per construct, wherein colours indicate two independent experiments. Source data are provided as a Source data file.
