Fig. 3: Visualization of cDC2-tumour cell interaction.
From: The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culture
a Section of OMC showing multiplex fluorescence immunohistochemistry representative area. cDC2s (CD45+ cells, green) are found in close proximity to melanoma cells (tumour (tyrosinase and SOX10)+ cells, yellow) and fibroblasts (FAP+ cells, magenta). DAPI (blue) indicates nuclei (n = 2). b PKH26 (yellow) and second harmonic generation (SHG, cyan) signals of multiphoton images, acquired with excitation wavelength (λ) of 950 nm. PKH26 signal indicates cDC2s; SHG was generated by collagen bundles. c Characteristic amoeboid behaviour of DCs patrolling the environmental niche. d Representative time points during time-lapse recording of melanoma BLM cells expressing GFP [λ (excitation) = 950 nm]. BLM cells showed high cellular dynamics (dotted lines; protrusion, white arrowhead and retraction, blue arrow). e, f Representative time points during time-lapse recording of cDC2s (PKH26)-tumour cell (GFP) interaction [λ (excitation) = 950 nm]. e DC (dotted line, white) sensed and sampled tumour-derived particle (dotted line, yellow). f Prolonged interaction of DCs with tumour-derived fragments. Tumour cells showed intense membrane dynamics and blebbing. Scale bars, 20 μm.
