Fig. 4: Identification and phenotypic characterization of CD14⁺ DCs isolated from the human OMC.

a Two days after cDC2s injection, OMCs were digested and cell suspensions were stained. Representative flow cytometry contour plots showing gated cDC2s (CD1c⁺CD14⁻ cells) and CD14⁺ DCs (CD1c⁺CD14⁺ cells) in OSCs (upper contour plots) and OMCs (lower counter plots). Results obtained with three different melanoma cell lines (BLM, Mel624, A375) are shown. Numbers indicate the percentage of gated cells. b Pie charts depicting the frequency of cDC2s and CD14⁺ DCs in OSCs and OMCs. Independent experiments were performed with three different melanoma cell lines: BLM (n = 8), Mel624 (n = 2), A375 (n = 2). Frequencies from control OSCs are pooled (n = 12). Data are mean ± SD. c Graphs showing the frequency of cDC2s (p = 0.0005) and CD14⁺ DCs (p = 0.0005) in OSCs and OMCs (across the three melanoma cell lines). A non-parametric two-tailed Wilcoxon signed-rank test was used for statistical analysis. d Representative overlaid histogram plots showing expression of the indicated markers in cDC2s and CD14⁺ DCs isolated from OMCs. For each marker a summary graph reporting the ratio of the geometric mean of the fluorescence intensity (GeoMFI) between CD14⁺ DCs and cDC2s isolated from OSCs and OMCs is shown. A blue background indicates a GeoMFI ratio <1, higher expression in cDC2s; whereas a red background indicates a GeoMFI ratio >1, higher expression in CD14⁺ DCs. At least n = 3 donors for each marker are included. The p values were estimated using a non-parametric two-tailed Wilcoxon signed-rank test. e Representative histogram plots and GeoMFI graphs of macrophage-related markers CD163, CD206 and MerTK in cDC2s and CD14⁺ DCs isolated from OMCs. At least n = 3 donors for each marker are provided. The p values were estimated using a non-parametric two-tailed Wilcoxon signed-rank test. Statistical significance was annotated as follows: *p < 0.05, ***p < 0.001. Source data are provided as a Source data file.