Fig. 7: CD14⁺DCs in melanoma lesion phenotypically resemble those recapitulated in human OMC.

Human metastatic melanoma lesions were digested and stained (n = 3). a CD1c⁺CD14⁻ (cDC2s) and CD1c⁺CD14⁺ (CD14⁺ DCs) were identified and gated based on CD1c and CD14 expression in CD45⁺CD1c⁺ live single cells. A representative contour plot from one patient (Pt. 1) is shown. b Pie charts report percentages (mean ± SD) of cDC2s and CD14⁺ DCs across different melanoma patients (n = 3, in triplicate samples; mean ± SD). c Graphs showing GeoMFI’s of the phenotypic analysis of cDC2s, CD14⁺ DCs and CD14⁺ mono/macs defined within intra-tumoural CD45⁺CD11c⁺ live single cells from melanoma patient samples. Representative histogram plots for each indicated marker are shown. Colour legends indicate: cDC2s (orange), CD14⁺ DCs (green), CD14⁺ mono/macs (red). Source data are provided as a Source data file.