Fig. 8: Analysis of patient-derived OMC.

a, b Comparison of melanoma patient’s cell suspension cultured in a de-epidermized human dermis versus ex vivo analysis. Two days after injection, microtissues containing the patient material were fixed and processed for paraffin embedding. a Multiplex IHC staining on tissue sections was performed using the following antibodies: CD3 (red), CD8 (light blue) and tumour marker (white). The latter consists of a mix of antibodies recognizing the melanoma antigens: HMB45 (gp100), MelanA (MART-1), Tyrosinase and SOX10. Representative H&E stain and multicolour composite pictures for T-cell-related antibodies (T-cell panel) are shown (n = 1, Pt. 3). Scale bar, 50 µm. b Cytometric image manual quantification analysis of multiplex IHC sections (n = 7) shown in panel (a), to identify lymphocytes (tumour marker-CD3⁺CD8⁺ and Tumour marker-CD3⁺CD8⁻ events). Mean ± SEM; two-tailed unpaired t tests) (p < 0.0001) (Gating strategy is shown in Supplementary Fig. 11). c, d Representative IHC images of tumour-specific characteristics in original melanoma lesion (left) and pt-derived OMC (right) (n = 3). Scale bars, 100 μm. d Overview of tumour-specific characteristics in original melanoma lesions and pt-derived OMCs. Ctrl is control melanoma tissue. e Graphs reporting the Geometric mean of fluorescence intensity (GeoMFI) of the indicated markers in CD14⁺ monocytes cultured within OSCs or pt-derived OMCs (n = 3; except for PD-L1: n = 2). Source data are provided as a Source Data file.