Fig. 3: Chromatin structure and replication differs between micronuclei and primary nuclei. | Nature Communications

Fig. 3: Chromatin structure and replication differs between micronuclei and primary nuclei.

From: Supernumerary B chromosomes of Aegilops speltoides undergo precise elimination in roots early in embryo development

Fig. 3

a Micronuclei chromatin assessed by the TUNEL assay. 9% (n = 184) of analyzed micronuclei (arrowed) were TUNEL positive (in green). Scale bar, 10 µm. b None of the micronuclei (n = 120) incorporated EdU, despite normal DNA replication of the primary nuclei. EdU is shown in purple, the B-specific FISH probe AesTR-183 in green. Scale bar, 10 µm. c–f Electron micrographs of ultra-thin tissue sections of developing +B embryos. Micronuclei (n = 30) are marked by red squares and are further enlarged. c, d A micronucleus with a disrupted membrane (arrowed, was observed in 20% of the cases) and an increased chromatin density (43% of micronuclei showed dense chromatin) in comparison to the corresponding nucleus. Scale bar, 1 µm. e, f Micronucleus surrounded by an intact double membrane and with a considerable amount of euchromatin. Scale bar, 1 µm.

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