Fig. 2: Newly synthesized neutrophils upon anti-Ly6G have membrane Ly6G paucity.

a Old (>20 weeks) C57BL/6 mice were treated with anti-Ly6G 50 μg daily for 9 days. After sampling, bone marrow and blood cells were re-incubated with 10 μg of anti-Ly6G to saturate the Ly6G antigen, and subsequently stained with a donkey anti-rat PE-labelled antibody to measure the whole amount of Ly6G antigen at the surface of neutrophils. MFI, mean fluorescence intensity. RNA levels for Ly6g on purified neutrophils from the same mice is also shown in the right panel. n = 4 control (Ctr) and 5 anti-Ly6G mice. b (left) Setting for experiments performed on C57BL/6 mice of >20 weeks. (right) Prevalence of CD45⁺CD11b⁺Ly6G^intra+ neutrophils among total CD45⁺ immune cells and their respective BrdU-60h incorporation. c Neutrophil segmentation, which correlates with neutrophil aging, was analyzed on Giemsa-stained blood smears. n = 5 control (Ctr) and 4 treated mice. All the results from (b, c) were acquired from the same blood samples. *p < 0.05, from Mann–Whitney test; error bars represent s.d. Source data are provided as a Source Data file.