Fig. 3: Rationale for an optimized anti-Ly6G-based “Combo” depletion strategy.
From: Durable and controlled depletion of neutrophils in mice
a Scheme introducing the “isotype switch” strategy to enhance the anti-Ly6G killing celerity. b Experimental plan to evaluate the impact of anti-antibodies on the ability of anti-Ly6G to bind the Ly6G antigen. Neutrophils from blood were gated as CD45+CD11b+Ly6Gintra+. The MFI of Ly6G extracellular was then measured. One can see that both anti-IgG immunized mice or mice treated with anti-Ly6G + anti-rat (MAR 18.5) antibodies have more Ly6G antigens available 1.5 days after anti-Ly6G injection. c According to the data obtained in (b), the ideal scheme for double antibody-based neutrophil depletion requires a daily injection of anti-Ly6G. *p < 0.05, **p < 0.01 from Mann–Whitney test; error bars represent s.d. 5–7 control (Ctr) and 4–5 treated mice were used per group. Source data are provided as a Source Data file.
