Fig. 5: Enhancer-promoter interactions are formed progressively during erythroid differentiation and correlate with upregulation of gene expression.
From: Dynamics of the 4D genome during in vivo lineage specification and differentiation
Quantification of enhancer-promoter interactions and gene expression during in vivo erythroid differentiation at six erythroid gene loci. Contact frequencies (black circles; left Y-axis) represent unique interactions normalized for the total number of contacts in the matrix. Expression counts (gray squares; right Y-axis) represent mean expression in counts per million (CPM) for each population as determined by single-cell RNA-seq. Expression of α-globin, Slc25a37 and Tal1, is upregulated early in differentiation, concomitant with increased enhancer-promoter interactions. Cd47 is expressed in hematopoietic stem cells and further upregulated later in erythroid differentiation when enhancer-promoter interactions are strengthened. Cpeb4 and Btg2 become robustly expressed in the S1 stage and are further upregulated as enhancer-promoter interactions increase later in differentiation.
