Fig. 1: Tumour growth and angiogenesis are enhanced in pdgfrβcre+;fakfl/fl mice.

a B16F0 melanoma and Lewis Lung Carcinoma (LLC) subcutaneous tumour growth was increased in pdgfrβcre+;fakfl/fl mice compared with pdgfrβcre-;fakfl/fl mice. Images of representative tumours. Data show mean ± s.e.m. n = 17 (B16F0) and 7 (LLC) pdgfrβcre-;fakfl/fl and 25 (B16F0) and 8 (LLC) pdgfrβcre+;fakfl/fl mice per tumour type. **p = 0.009. Scale bar, 500 μm. b Spontaneous pancreatic insulinoma tumour volume is increased in RIP-Tag;pdgfrβcre+;fakfl/fl mice compared with RIP-Tag;pdgfrβcre-;fakfl/fl mice. Chart represents mean total macroscopic tumour volume ± s.e.m. n = 9 RIP-Tag;pdgfrβcre-;fakfl/fl and 11 RIP-Tag;pdgfrβcre+;fakfl/fl mice. **p = 0.0042. Representative images of pancreatic tumours stained for SV40. Scale bar, 0.5 cm. c Tumour blood vessel density is increased in tumours grown in pdgfrβcre+;fakfl/fl compared with pdgfrβcre-;fakfl/fl mice. Charts represent mean ± s.e.m. n = 3 pdgfrβcre-;fakfl/fl and 3 pdgfrβcre+;fakfl/fl B16F0 tumours, **p = 0.009; 19 pdgfrβcre-;fakfl/fl and 12 pdgfrβcre+;fakfl/fl LLC tumours *p = 0.0207; 7 RIP-Tag;pdgfrβcre-;fakfl/f and 10 RIP-Tag;pdgfrβcre+;fakfl/fl tumours, **p = 0.0097. Representative images of endomucin stained sections. Scale bar for B16F0 and LLC tumour sections, 200 µm. Scale bar for RIP-Tag tumour sections, 300 µm. d Pericyte association with tumour blood vessels is reduced in pdgfrβcre+;fakfl/fl and RIP-Tag;pdgfrβcre+;fakfl/fl mice. Charts represent the percentage of α-SMA positive blood vessels ± s.e.m. n = 5 pdgfrβcre-;fakfl/fl, 6 pdgfrβcre+;fakfl/fl, 10 RIP-Tag;pdgfrβcre-;fakfl/fl, 9 RIP-Tag;pdgfrβcre+;fakfl/fl mouse tumours. **p = 0.0043 (B16F0), **p = 0.003 (RIP-Tag). e Blood vessel leakage was similar in tumours from pdgfrβcre-;fakfl/fl and pdgfrβcre+;fakfl/fl mice. Chart shows Hoechst area relative to blood vessel area ± s.e.m. n = 25 high power fields in tumours from 4 pdgfrβcre-;fakfl/fl mice and 34 fields in tumours from 4 pdgfrβcre+;fakfl/fl mice. f Blood vessel associated endothelial cells from pdgfrβcre+;fakfl/fl mice have increased proliferation. Lower panels show representative high power images of insert Ki67, DAPI, endomucin. Chart represents the percentage of proliferating endothelial cells per area ± s.e.m., n = 24 and 27 fields in 4 pdgfrβcre-;fakfl/fl, 5 pdgfrβcre+;fakfl/fl mouse tumours respectively. *p = 0.0457. a–e two-sided Mann–Whitney U-test; c, d, f Two-sided Student’s t-test; ns not significant. Source data are provided as a Source data file.