Fig. 1: TVM cells have high SRC and CIV, which increases with age. | Nature Communications

Fig. 1: TVM cells have high SRC and CIV, which increases with age.

From: Metabolic characteristics of CD8+ T cell subsets in young and aged individuals are not predictive of functionality

Fig. 1

a Oxygen consumption rate (OCR) across time for sorted TN, TVM and TMEM cells from the spleens of naive young and aged SPF mice. Arrows indicate the addition of mitochondrial inhibitors (oligomycin; FCCP; antimycin A/rotenone) or timepoints for assessment of SRC (OCRBas, OCRMax) (n = 2–4, 5 experimental replicates). b Change in OCR from OCRBas to OCRMax (SRC) for each sorted subset (n = 2–4, 5 experimental replicates). c Electron microscope images of sorted cells directly ex vivo, scale bar indicates 0.2 µm (1 experimental replicate). d Confocal microscopy of sorted cells directly ex vivo, green fluorescence is Cytochrome C staining, scale bar indicates 2 µm, which was used to define (e), predominant mitochondrial morphology (fused, intermediate or fragmented) for 140 cells per subset and f average mitochondrial footprint per cell as calculated from confocal images (3 experimental replicates). g BN-PAGE and Blot for Cox5a from ETC CIV, with bands for CIV, CII, CIII2, CV and the CI/CIII2/CIV supercomplex indicated, alongside the Coomassie stained blot (3 experimental replicates). h Oxygen consumption rate (OCR) across time for sorted TVM cells from young mice, with high (200 µM) or low (5 µM) dose Etomoxir, (n = 3, 3 experimental replicates). Shown is mean ± standard error of the mean (SEM). NS indicates not significant, * indicates p ≤ 0.05, ** indicates p ≤ 0.01, unpaired t test.

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