Fig. 3: T_VM cells comprise the majority of the CD44^hiCD62L^hi T_CM cell population.

a Histograms for CD49d expression on CD44^hiCD62L^hi CD8⁺ T cells (T_CM cells) from naive young mice, naive aged mice and young mice after infection with LCMV (40 days post infection), with bar graphs showing the proportion of T_CM cells that are T_VM cells (n = 4–5). b Overlays of CD44^hiCD49d^lo T_VM cells on total CD8⁺ T cells with T_EM/T_CM cell gating (CD44^hiCD62L^lo and CD44^hiCD62L^hi, respectively). c Overlays of CD44^hiCD49d^hi T_MEM cells on total CD8⁺ T cells. d Representative dot plots identifying IAV-specific tetramer⁺ CD8⁺ T cells that are CD62L^hi (T_CM cells) or CD62L^lo (T_EM cells) (60 days post infection), with bar graphs of the average frequency of tetramer⁺ CD8⁺ T cells that are in each subset (n = 5). e OCR for sorted T_N and T_VM cells from young uninfected mice and sorted T_CM and T_EM cells from LCMV-infected mice (60 days post infection) and f change in OCR for each sorted subset (n = 5–6, 3 experimental replicates). Data from a–d are representative of at least 2 individual experiments. Shown is mean ± SEM. * indicates p ≤ 0.05, ** indicates p ≤ 0.01, unpaired t test.