Fig. 5: High IL-15 sensitivity in TVM cells increases with age and mediates increased SRC. | Nature Communications

Fig. 5: High IL-15 sensitivity in TVM cells increases with age and mediates increased SRC.

From: Metabolic characteristics of CD8+ T cell subsets in young and aged individuals are not predictive of functionality

Fig. 5

a IL-15Rβ MFI directly ex vivo from individual mice (n = 5–7) or b pSTAT5 MFI after 15 min of stimulation with IL-15 in vitro on sorted TN, TVM and TMEM cells from young or aged mice (n = 3). c Representative dot plots for CD8+ T cells gated on TN (CD44lo), TVM (CD44hiCD49dlo) and TMEM (CD44hiCD49dhi) cells (frequency of CD8+ T cells indicated) and d frequency of CD8+ T cells in each subset in WT or IL-15 KO mice (n = 5 for WT and 7 for KO). e OCR for sorted TN, TVM and TMEM cells from young uninfected, IAV-infected and IAV-infected/IL-15 neutralising mAb treated mice and f change in OCR for each sorted subset. Shown is mean ± SEM. * Indicates p ≤ 0.05, ** indicates p ≤ 0.01, unpaired t test, data for a, b, e, f are representative of at least 3 individual experiments.

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