Fig. 3: Near absence of 24-nt phasiRNAs in CRISPR-derived dcl5 mutant alleles.
From: Dicer-like 5 deficiency confers temperature-sensitive male sterility in maize
a Total 24-nt phasiRNAs from 176 24-PHAS loci are highly abundant in both 1.5 and 2.0 mm fertile heterozygous anthers, but abundance is reduced by >99% in dcl5-1, dcl5-2, and dcl5-4, and by >90% in dcl5-3 anthers. Altered temperature regimes do not restore 24-nt phasiRNA abundances in either fertile (permissive) or sterile (restrictive) dcl5-1 anthers (see main text). W23 inbred fertile anthers have similar 24-nt phasiRNA abundance under both temperature regimes. Error bars indicate standard error of the mean (s.e.m.). b, inset Representative 24-PHAS locus #12 (B73, v4; chr 1, 178454619 bp) in a genome browser showing ~1.5 kbp with sRNAs in the fertile heterozygote (left) and dcl5-1 (right). Orange dots are individual 24-nt phasiRNAs; x-axis is genome position on the top or bottom strand, and the y-axis depicts abundance in each genotype. Other colored dots are as indicated in the key, lower right.
