Fig. 3: Coverage and enrichment across fusion-positive cancer cell lines. | Nature Communications

Fig. 3: Coverage and enrichment across fusion-positive cancer cell lines.

From: Partner independent fusion gene detection by multiplexed CRISPR-Cas9 enrichment and long read nanopore sequencing

Fig. 3

a Mean coverage and b enrichment across the genome, on-target (cut to breakpoint), BP-spanning (breakpoint-spanning), and fusion-spanning (across the fusion junction) for the three cell lines A4573, CHP-100, and HS-SYII. c Coverage plots for the cell line A4573 for the two fusion partners EWSR1 (targeted) and FLI1. d Coverage plots for the cell line CHP-100 for the two fusion partners EWSR1 (targeted) and FLI1. e Coverage plots for the cell line HS-SYII for the two fusion partners SS18 (targeted) and SSX1. Dotted lines (green) indicate the crRNA-directed Cas9 cleavage positions and dashed lines (red) indicate breakpoint positions. Arrows indicate the directionality of reads created from the specific crRNA design. Source data are provided as a Source Data file.

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