Fig. 4: Fusion gene coverage and enrichment from tumor samples. | Nature Communications

Fig. 4: Fusion gene coverage and enrichment from tumor samples.

From: Partner independent fusion gene detection by multiplexed CRISPR-Cas9 enrichment and long read nanopore sequencing

Fig. 4

a Coverage plots for the RH tumor sample for the two fusion partners PAX3 (targeted) and FOXO1. PAX3 was targeted with three sequential guides to span the 18 kb possible breakpoint region. b Coverage plots for the CML tumor sample for the two fusion partners BCR (targeted) and ABL1. BCR was targeted with eleven sequential guides to span the possible breakpoint region. c Coverage plots for the ALL2 tumor sample for the deletion between CRLF2 (targeted) and P2RY8 (targeted). d Mean coverage and e mean enrichment across the genome, on-target (cut to breakpoint), BP-spanning (breakpoint-spanning), and fusion-spanning (across the fusion junction) for the tumor samples ES1, RH, CML, BL, ALL1, and ALL2. Dotted lines (green) indicate the crRNA-directed Cas9 cleavage positions and dashed lines (red) indicate breakpoint positions. Arrows indicate the directionality of reads created from the specific crRNA design. Source data are provided as a Source Data file.

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