Fig. 6: Fusion gene detection from WGA tumor DNA. | Nature Communications

Fig. 6: Fusion gene detection from WGA tumor DNA.

From: Partner independent fusion gene detection by multiplexed CRISPR-Cas9 enrichment and long read nanopore sequencing

Fig. 6

a Mean coverage and b enrichment across the genome, on-target (cut to breakpoint), BP-spanning (breakpoint-spanning), and fusion-spanning (across the fusion junction) for the whole genome amplified tumor material C1 and C2. c Coverage plots for the whole genome amplified tumor material C1 for the two fusion partners AGAP3 and BRAF (targeted). d Coverage plots for the whole genome amplified tumor material C2 for the two fusion partners TRIM24 and BRAF (targeted). Dotted lines (green) indicate the crRNA-directed Cas9 cleavage positions and dashed lines (red) indicate breakpoint positions. Arrows indicate the directionality of reads created from the specific crRNA design. Source data are provided as a Source Data file.

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