Fig. 1: Leucine deprivation induces WAT browning. | Nature Communications

Fig. 1: Leucine deprivation induces WAT browning.

From: Activation of GCN2/ATF4 signals in amygdalar PKC-δ neurons promotes WAT browning under leucine deprivation

Fig. 1

a Daily food intake. b Fat mass by NMR. c Subcutaneous WAT (sWAT) weight. d Representative images of hematoxylin and eosin (H&E) staining of sWAT. e sWAT cell size quantified by Image J analysis of H&E images. f Gene expression of Ucp1, Pgc1a, Cidea, Dio2, and Prdm16 in sWAT by RT-PCR. g Representative images of immunohistochemistry (IHC) staining of UCP1 in sWAT. h UCP1 protein in sWAT by western blotting (top) and quantified by densitometric analysis (bottom); A.U.: arbitrary units. Studies were conducted using 14- to 15-week-old male wild-type mice fed a control (Control) or leucine-deficient [(-) L] diet for 3 days. Data are expressed as the mean ± SEM (n represents number of samples and are indicated above the bar graph), with individual data points. Data were analyzed by two-tailed unpaired Student’s t test. Source data are provided as a Source data file.

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