Fig. 2: Silencing PKC-δ neuronal activity blocks WAT browning under leucine deprivation.

a Immunofluorescence (IF) staining for tdTomato (red), c-Fos (green) and merge (yellow) in central amygdala (CeA) sections (left), and quantification of c-Fos and tdTomato colocalized cell numbers (right). b Daily food intake. c Fat mass by NMR. d Subcutaneous WAT (sWAT) weight. e Representative images of hematoxylin and eosin (H&E) staining of sWAT. f sWAT cell size quantified by Image J analysis of H&E images. g Gene expression of Ucp1, Pgc1a, Cidea, Dio2, and Prdm16 in sWAT by RT-PCR. h Representative images of immunohistochemistry (IHC) of UCP1 in sWAT. i UCP1 protein in sWAT by western blotting (left) and quantified by densitometric analysis (right); A.U.: arbitrary units. Studies for a were conducted using 12- to 14-week-old male PKC-δ-Cre-Ai9 mice fed a control (Control) or leucine-deficient [(-) L] diet for 3 days; studies for b–i were conducted using 22- to 24-week-old male PKC-δ-Cre mice receiving AAVs expressing mCherry (PKC δ − hM4Di) or hM4Di (PKC δ + hM4Di), all received CNO injections every 12 h for 3 days, simultaneously fed a Control or (-) L diet for 3 days. Data are expressed as the mean ± SEM (n represents number of samples and are indicated above the bar graph), with individual data points. Data were analyzed by two-tailed unpaired Student’s t test for a, or by one-way ANOVA followed by the SNK (Student–Newman–Keuls) test for b–i. Source data are provided as a Source data file.