Fig. 4: Knockdown of GCN2 in amygdalar PKC-δ neurons blocks leucine deprivation-induced WAT browning. | Nature Communications

Fig. 4: Knockdown of GCN2 in amygdalar PKC-δ neurons blocks leucine deprivation-induced WAT browning.

From: Activation of GCN2/ATF4 signals in amygdalar PKC-δ neurons promotes WAT browning under leucine deprivation

Fig. 4

a Immunofluorescence (IF) staining for tdTomato (red), p-GCN2 (green) and merge (yellow) in central amygdala (CeA) sections (left), and quantification of p-GCN2 and tdTomato colocalized cell numbers (right). b Daily food intake. c Fat mass by NMR. d Subcutaneous WAT (sWAT) weight. e Representative images of hematoxylin and eosin (H&E) staining of sWAT. f sWAT cell size quantified by Image J analysis of H&E images. g Gene expression of Ucp1, Pgc1a, Cidea, Dio2, and Prdm16 in sWAT by RT-PCR. h Representative images of immunohistochemistry (IHC) of UCP1 in sWAT. i UCP1 protein in sWAT by western blotting (left) and quantified by densitometric analysis (right); A.U.: arbitrary units. Studies for a were conducted using 12- to 14-week-old male PKC-δ-Cre-Ai9 mice fed a control (Control) or leucine-deficient [(-) L] diet for 3 days; studies for b–i were conducted using 13- to 16-week-old male PKC-δ-Cre mice receiving AAVs expressing GFP (PKC δ − shGCN2) or shGCN2 (PKC δ + shGCN2) fed a Control or (-) L diet for 3 days. Data are expressed as the mean ± SEM (n represents number of samples and are indicated above the bar graph), with individual data points. Data were analyzed by two-tailed unpaired Student’s t test for a, or one-way ANOVA followed by the SNK (Student–Newman–Keuls) test for b–i. Source data are provided as a Source data file.

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