Fig. 2: Binding of nucleosides and related molecules to CxD7L1 by ITC. | Nature Communications

Fig. 2: Binding of nucleosides and related molecules to CxD7L1 by ITC.

From: ADP binding by the Culex quinquefasciatus mosquito D7 salivary protein enhances blood feeding on mammals

Fig. 2

Binding experiments were performed on a VP-ITC microcalorimeter. Assays were performed at 30 °C. The upper curve in each panel shows the measured heat for each injection, while the lower graph shows the enthalpies for each injection and the fit to a single-site binding model for calculation of thermodynamic parameters. Titration curves are representative of two measurements. Panels (a–e) show adenine nucleosides or nucleotides that bind CxD7L1: adenosine 5-triphosphate (a), adenosine 5-diphosphate (b), adenosine 5-monophosphate (c), adenosine (d), and adenine (e). In panels (f–j), other purine and pyrimidine nucleotides and related substances showed no binding to CxD7L1: guanosine 5-triphosphate (f), thymidine 5-triphosphate (g), adenosine 3-monophosphate (h), cyclic adenosine monophosphate (i), and polyphosphate (j). The insets show the names and chemical formulas for these compounds.

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