Fig. 7: Effect of CxD7 proteins on platelet aggregation induced by secondary mediators. | Nature Communications

Fig. 7: Effect of CxD7 proteins on platelet aggregation induced by secondary mediators.

From: ADP binding by the Culex quinquefasciatus mosquito D7 salivary protein enhances blood feeding on mammals

Fig. 7

Prior to the addition of the agonist, platelet-rich human plasma was incubated for 1 min either with PBS (Crtl) or with the recombinant proteins, or SQ29,548 at the concentrations shown. Aggregometer traces were measured at 37 °C from stirred platelets suspensions on a Chrono-Log platelet aggregometer model 700 for 6 min. An increase of light transmittance over time indicates platelet aggregation. a Platelet aggregation traces using different concentrations of ADP (0.5, 1, and 10 µM) as aggregation agonist. b Platelet aggregation traces using 1 µM U-46619, 0.125 µM arachidonic acid (AA), or low collagen concentration (1 µg/mL). Graphs are representative of two measurements. Source data are provided as a Source Data file.

Back to article page