Fig. 1: 5S rRNA in the ribosome.
From: Ribosome engineering reveals the importance of 5S rRNA autonomy for ribosome assembly
a Secondary structure of the 5S rRNA; RNA stems (I-V) and loops (A–E) are indicated. b The view of the E. coli 50S ribosomal subunit from the interface side (PDB 4YBB7). 5S rRNA is shown in red, 23S rRNA is light blue and r-proteins in dark blue. c Interactions between wt 5S rRNA and 23S rRNA (as seen from the large subunit solvent side). The native ends of the 5S rRNA and the nearest accessible 23S rRNA site are marked by golden spheres. Loops C and D, which are in a close proximity to 23S rRNA, are indicated. Proteins are removed for clarity. d Engineering cp5S rRNA by linking its native ends with a tetra-loop. The altered/added residues are in green. e The cp5S rRNA integration sites. The shortest distances between the nucleotide phosphates of the 5S rRNA and 23S rRNA are indicated by dotted lines.
