Fig. 4: Functional ribosomes with the hybrid 23S-cp5S rRNA are highly active in translation.
From: Ribosome engineering reveals the importance of 5S rRNA autonomy for ribosome assembly
a In vitro translation of dihydrofolate reductase (DHFR) protein by wt ribosomes or23S-cp5S rRNA DH42* ribosomes. Top: SDS–gel analysis of [35S]-labeled DHFR protein synthesized in the cell-free translation system. The intensities of the full-size protein bands were plotted in the graph shown below the gel (mutant—blue curve, wt—black curve). b Time course of in vitro expression of sfGFP protein by wt or mutant ribosomes as evaluated by fluorescence (mutant—blue curve, wt—black curve). Graphs shown in a and b represent the average of three independent experiments with error bars indicating s.d. The uncropped gels and raw data can be found in the Source data file.
