Fig. 6: Structure of the active ribosomes with the hybrid 23S-cp5S rRNA.
From: Ribosome engineering reveals the importance of 5S rRNA autonomy for ribosome assembly
a Cryo-EM reconstruction of the 23S-cp5S rRNA 70S ribosome with A and P sites occupied by tRNAs (green). 23S rRNA-linked cp5S rRNA is shown in red. b Fitting of tethered cp5S rRNA in the cryo-EM density. cp5S rRNA is red, tethers are purple and 23S rRNA nucleotides are light-blue. Inlet shows the modeled positions of nucleotides involved in forming the cp5S - 23S rRNA junction (see also Supplementary Fig. 4a). c The path of the rRNA backbone in wt ribosomes (left panel)67 and in ribosomes with hybrid 23S-cp5S rRNA (right panel) deduced by cryo-EM analysis. Colors are as in b. d, e Tethering 23S and cp5S rRNA introduces minimal perturbation at the junction site with only G1026 and A1027 at the site of attachment (c) and a more distant A1134 (d) undergoing conformational transitions. The orientation of the 23S rRNA residues in the wt ribosomes is shown in gray, 23S rRNA nucleotides of the mutant ribosome are light blue and the RNA residues belonging to the 23S rRNA-cp5S rRNA tethers are purple.
