Fig. 5: Model describing the role of DNA strain in RNA polymerase III initiation complexes. | Nature Communications

Fig. 5: Model describing the role of DNA strain in RNA polymerase III initiation complexes.

From: DNA origami-based single-molecule force spectroscopy elucidates RNA Polymerase III pre-initiation complex stability

Fig. 5

On the U6 snRNA promoter, the −1 nucleosome is firmly positioned close to the upstream promoter region, limiting DNA flexibility. Continuous transcription of the U6 snRNA by promoter-bound RNA polymerase III creates torsional strain. The +1 nucleosome is positioned downstream of the gene body. Proteins and nucleic acids are colour coded as follows: TBP (yellow), Brf2 (orange), Bdp1 (red), RNA polymerase II/III (grey), nucleosome (brown), template strand (blue) and non-template strand (cyan).

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