Fig. 2: Deconvolved cell type distributions are consistent with SST and PVALB single marker expression maps.

Using CibersortX²², frontal and visual cortex snDrop-Seq data from Lake and colleagues³¹ were used to deconvolve cell type fractions from bulk AHBA microarray expression data. Deconvolved cell fractions of a somatostatin and b parvalbumin interneurons across cortex using single-cell data from frontal (left) and visual (right) cortex. c Somatostatin and parvalbumin cell fraction maps were z-transformed and subtracted (SST–PVALB) to illustrate the relative density of each subtype across cortex. d Spatial correlations of each deconvolved cell type across cortex using frontal (top-left triangle) and visual (bottom-right triangle) cell signatures. e Example marker genes for each cell class. f Deconvolved parvalbumin fractions across cortex were positively spatially correlated (Pearson’s) to single-gene PVALB expression (frontal cortex: r(337) = 0.81, p < 2.2e−16; visual cortex: r(337) = 0.48, p < 2.2e−16). g Deconvolved somatostatin fractions across cortex are positively spatially correlated (Pearson’s) to single-gene SST expression (frontal cortex: r(337) = 0.72, p < 2.2e−16; visual cortex: r(337) = 0.60, p < 2.2e−16).