Fig. 2: SPK-localization dependencies and complex formation. | Nature Communications

Fig. 2: SPK-localization dependencies and complex formation.

From: Spitzenkörper assembly mechanisms reveal conserved features of fungal and metazoan polarity scaffolds

Fig. 2

a The localization of the indicated mGFP-tagged proteins was determined by confocal microscopy in the indicated strains. Dotted white lines show the hyphal outline. Scale bar = 10 μm. b Native PAGE identifies two stable hetero-oligomeric complexes formed by JNS-1 and SPA-2 (HO-1 and HO-2). This figure is related to Supplementary Fig. 1. c SPA-2-mGFP precipitates SPA-2-HA in the absence of JNS-1 (left panels), and JNS-1-mGFP precipitates JNS-1-HA in the absence of SPA-2 (right panels). d SPA-2 and JNS-1 depend on each other for interaction with SPZ-1. The signal from mass spectrometry is compared to the wild type (WT) precipitation according the scale shown in the legend. Source data are provided as a Source Data file.

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