Fig. 3: Functional dissection of SPZ-1, JNS-1 and SPA-2.

a Conservation, coiled-coil, and coiled-coil dimer probability is shown for SPZ-1. The indicated regions of SPZ-1 (r1–r7) were replaced by an mCherry-selectable marker fusion as described in materials and methods. Functionality of the variants is scored according to the color scale, where 1 and 0 represent wild type and deletion mutant growth rates, respectively. This figure is related to Supplementary Fig. 2a. b Localization of the indicated SPZ-1 deletion variants. Dotted white lines show the hyphal outline. Scale bar = 10 μm. c The ability of the indicated SPZ-1 variants to co-precipitate interacting proteins is compared to full-length SPZ-1 (WT). The signal from mass spectrometry is compared to WT according the scale shown in the legend. If the signal from mass spectrometry exceeds that of the WT, this value is identified with white numbers. d Conservation, coiled-coil and coiled-coil dimer prediction for JNS-1. The indicated regions of JNS-1 (r1-r3) were replaced by an mCherry-selectable marker fusion as described in materials and methods. Variant functionality is indicated as in a. This figure is related to Supplementary Fig. 2b. e Localization of the indicated JNS-1 deletion variants. Dotted white lines show the hyphal outline. Scale bar = 10 μm. f Native PAGE analysis of SPA-2 mobility in the indicated JNS-1 deletion variants. g Conservation, coiled-coil and coiled-coil dimer probability for SPA-2. The indicated regions of SPA-2 (r1–r5) were replaced by an mCherry-selectable marker fusion as described in materials and methods. Variant functionality is indicated as in (a). This figure is related to Supplementary fig. 2c. h Native PAGE analysis of JNS-1 mobility in the indicated SPA-2 deletion variants. i Localization of the indicated SPA-2 deletion variants. Dotted white lines show the hyphal outline. Scale bar = 10 μm. j The ability of the indicated SPA-2 variants to co-precipitate interacting proteins is compared to full-length SPA-2 (WT). The mass spectrometry is quantified as in (c). Source data are provided as a Source Data file.