Fig. 6: Identification of LAH-2 effectors, and the influence of scaffolds on secretory markers. | Nature Communications

Fig. 6: Identification of LAH-2 effectors, and the influence of scaffolds on secretory markers.

From: Spitzenkörper assembly mechanisms reveal conserved features of fungal and metazoan polarity scaffolds

Fig. 6

a COT-1, MOB-2A and GPI-1 depend on LAH-2 for tip-localization. The indicated mGFP fusion proteins were combined with the indicted deletion strains and imaged by confocal microscopy. Note that the partial SPA-2 and JNS-1 dependence of the LAH-2 effectors is similar to that displayed by LAH-2 itself (see Fig. 2a). Dotted white lines show the hyphal outline. Scale bar = 10 μm. b Secretory vesicles and organelles do not depend on any of the scaffolds for SPK-localization. The indicated mGFP or mCherry fusion proteins were combined with the indicted deletion strains and imaged by confocal microscopy. Dotted white lines show the hyphal outline. Scale bar = 10 μm. c Co-expression of a marker of micro-vesicles (CHS-1) and macro-vesicles (GS-1) in the spz-1 deletion mutant reveals localization to inner and outer layers of the SPK, respectively. Wild-type (WT) accumulation is shown for comparison. Dotted white lines show the hyphal outline. Scale bar = 10 μm. Source data are provided as a Source Data file.

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