Fig. 7: A blueprint for assembly of the of SPK proteinaceous scaffold.

a The cartoon depicts scaffold assembly, MYO-5 dependent transport and effector recruitment. Parallel transport of secretory vesicles, and scaffold complexes is indicated with dashed orange arrows. Complexes are boxed. Secretory vesicles and F-actin are depicted according to the legend. The positive feedback from CCP-1 to F-actin is shown with a solid arrow from CCP-1 to an F-actin filament. The dependency of LAH-2 on CCP-1 is likely to be an indirect consequence of diminished SPK F-actin. This relationship is depicted with a dashed arrow from an F-actin filament to LAH-2. See discussion for additional information. b Phylogenetic distribution of proteins examined in this study. Filled and empty squares indicate the presence and absence of the indicated protein, respectively. Arrows to branch nodes identify the likely origin of the indicated proteins. Note that with a few exceptions, all the SHD-containing proteins co-occur with coiled-coil binding partners (asterisk). Squares with colored borders identify complex multicellular fungi: the Pezizomycotina (orange), Neolecta (blue) and the Agaricomycotina (purple). Amino acid substitution per site is shown according to the indicated greyscale, which represents the degree of divergence from the reference Pezizomycotina sequences (see Methods). In the case of Pea2 and PIX the reference group is the Saccharomycotina and Metazoans, respectively.