Fig. 3: Potential of CapsidCas13a as a tool for modifying bacterial flora. | Nature Communications

Fig. 3: Potential of CapsidCas13a as a tool for modifying bacterial flora.

From: Development of CRISPR-Cas13a-based antimicrobials capable of sequence-specific killing of target bacteria

Fig. 3

Programmed CapsidCas13a altered the composition of bacterial population. A mixed cell population was prepared by mixing E. coli NEB5α F′Iq (control) with equal numbers of NEB5α F′Iq expressing blaIMP-1 and mcr-2, respectively. The cell mixtures were then independently treated with blaIMP-1-targeting, mcr-2-targeting, and nontargeting CapsidCas13a. Note that each AMR gene-targeting CapsidCas13a reduced the corresponding target cell population. The percentage represents the mean of three biological replicates. Source data are available in the Source Data file.

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