Fig. 1: Oenocyte ROS homeostasis non-autonomously modulates cardiac function. | Nature Communications

Fig. 1: Oenocyte ROS homeostasis non-autonomously modulates cardiac function.

From: Impaired peroxisomal import in Drosophila oenocytes causes cardiac dysfunction by inducing upd3 as a peroxikine

Fig. 1

a Arrhythmia index of oenocyte-specific Cat (n = 9) and Sod1 (n = 13) knockdown flies (1-week-old). Ctrl genotype is PromE>attP40 (n = 16). b Representative images of ROS levels in dissected oenocytes from flies fed on normal diet (white bar) or 10mM paraquat (grey bar). All flies express mCD8::GFP under PromE-Gal4. Sod1 was specifically overexpressed in the oenocytes (Sod1OE). Scale bar: 20 µm. c Quantification of the percentage of DHE-positive staining in region of interest ROIs from 5 flies (nleft-right = 13, 8, 12, 16 ROIs). d Representative M-mode showing heart contraction in control and Sod1 overexpression flies fed on normal or 10mM paraquat food. Sod1 was expressed using the GeneSwitch PromEGS-Gal4 (+RU). Ctrl genotype is PromEGS>Sod1OE with no RU (−RU). e Arrhythmia index of control and oenocyte-specific Sod1 overexpression flies fed on normal or 10 mM paraquat diets (nleft-right = 17, 16, 19, 15 flies). f Arrhythmia index of control and fat body/gut-specific Sod1 (S106-Gal4>Sod1OE) overexpression flies fed on normal or 10 mM paraquat diets. Overexpression specifically in fat body and gut (nleft-right = 15, 18, 21, 17 flies). g Representative M-mode showing heart contraction in young (2 weeks, white bar) and old (6 weeks, purple bar) flies with or without oenocyte-specific Sod1 overexpression. Ctrl genotype is PromEGS>Sod1OE with no RU. h Arrhythmia index of control and oenocyte-specific Sod1OE flies at young and old ages (nleft-right = 17, 19, 14, 18 flies). Data are represented as mean ± SEM. P values are calculated using either two-way ANOVA (c, e, f, h) or one-way ANOVA (a), followed by Holm-sidak multiple comparisons. ns: not significant.

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