Fig. 6: Peroxisomal import function is disrupted in aged oenocytes.

a Representative images of anti-SKL immunostaining of young and aged wild-type oenocytes. Scale bar: 6.7 µm. b Quantification on the number of SKL-positive punctae in a. Dot plot shows the quantifications of 6 biological replicates, 2 ROIs per replicate. c Representative images to show co-localization of Pmp70 and YFP-PTS in young and aged oenocytes (Scale bar: 6.7 µm). Insets on the right show zoom-in peroxisome structures (the regions indicated by the white boxes in the merged panels, Scale bar: 125 nm). d Line scan analysis to show the fluorescence intensity of Pmp70 (red) and YFP-PTS (green) crossing peroxisomes in the insets (dashed line). e Quantification of the number of YFP-positive punctae normalized Pmp70 in c. N = 6. Dot plot shows the quantifications of 6 biological replicates, 4 ROIs per replicate. f Quantification of the number of Pmp70-positive punctae in c, N = 6 biological replicates, 4 ROIs per replicate. g Pearson correlation quantification measuring the correlation coefficiency of the co-localization between Pmp70 and YFP-PTS in c. N = 6 biological replicates. h Representative images of anti-SKL and anti-Pmp70 immunostaining of PromE^GS>Pex5^RNAi oenocytes. Scale bar: 6.7 µm. i Representative images of anti-SKL and anti-Pmp70 immunostaining of PromE^GS>Pex19^RNAi oenocytes. Scale bar: 6.7 µm. j Quantification of the number of SKL-positive punctae normalized Pmp70 in h, i. k Quantification of the number of Pmp70-positive punctae in h, i. l Quantification of Pearson correlation coefficiency of the co-localization between Pmp70 and SKL in h, i. 6 biological replicates, 3 ROIs per replicate. Data are represented as mean ± SEM. P values are calculated using two-sided unpaired t-test (b, e–g). or two-way ANOVA by Holm-Sidak multiple comparisons (j–l), ns: not significant. For specific statistical number, please refer to the source data.